Effect of dissolved oxygen regulation on the content of ganoderic acid S and R in mycelium of Ganoderma lucidum in a two-stage culture by oscillatory-stationary culture

Abstract

Abstract Currently, liquid fermentation is regarded as a feasible method for cultivating thyme as contrast to subentity culture. Nevertheless, the production of ganoderic acid S and R is still rather limited, thus requiring the need for parameter adjustment in the liquid fermentation process. The influence of thype, an oxygen bacterium, on the viability of microorganisms during liquid fermentation is significant. Moreover, liquid ferments can be classified into two distinct categories: oscillating and static. Prior studies have primarily focused on analyzing these two approaches separately, leading to discrepancies in the consistency and concentration of the fluid form and thyme content. Therefore, the primary objective of this study is to enhance the soluble parameter and incorporate both vibrating and static cultivation methods to facilitate the liquid erection of the grass. The experimental findings indicate that the manipulation of dissolved oxygen during the oscillation stage can significantly enhance the growth of Ganoderma lucidum biomass. However, its impact on the levels of ganoderic acids S and R is comparatively less pronounced. The manipulation of dissolved oxygen during the resting stage yielded contrasting outcomes, leading to a substantial increase in the levels of ganoderic acids S and R, while exerting a lower impact on biomass. The findings of this study demonstrated that the growth of G. lucidum primarily occurred during the oscillating culture stage, while the accumulation of ganoderic acid S and R was predominantly observed during the stationary culture stage. This approach introduces a novel concept for the liquid fermentation of G. lucidum, while simultaneously offering valuable insights for the industrial-scale production of ganoderic acid.