Affiliation:
1. Hospital Universitario Virgen de la Victoria
2. IMIBIC: Instituto Maimonides de Investigacion Biomedica de Cordoba
3. Universidad de Malaga
4. IDIS: Instituto de Investigacion Sanitaria de Santigao de Compostela
5. Instituto de Investigación Sanitaria La Fe: Instituto de Investigacion Sanitaria La Fe
Abstract
Abstract
This study hypothesized that investigating the promoter methylation of SFRP2 gene in various biological samples from patients with CRC could offer novel biomarker utility. Methods: The study examined SFRP2 gene expression and methylation in both healthy individuals and CRC patients. We assessed the comprehensive biomarker description value of SFRP2 in CRC, validated SFRP2 expression and methylation in diverse biological tissues, and evaluated its potential role as a biomarker in CRC. Furthermore, we investigated the effects of rhSFRP2 on cell proliferation, migration, and the expression of key genes associated with carcinogenesis and the Wnt pathway. RESULTS SFRP2 promoter methylation in whole blood significantly predicted cancer stage, lymph node invasion, and cancer recurrence in CRC patients (p<0.05). The global SFRP2 gene was found to be hypomethylated in CRC (p<0.001), and these results were validated in the TCGA-COAD and TCGA-READ cohorts. Promoter SFRP2 DNA methylation was responsive to chemotherapy, with treated CRC patients exhibiting lower SFRP2 methylation compared to untreated CRC patients (p<0.001). Low promoter SFRP2 methylation in untreated patients was linked to poor overall survival (p<0.05). In a functional analysis of a cell system, rhSFRP2 treatment in HCT116 cells restrained cell proliferation and migration, and led to the downregulation of the AXIN2 gene, which is implicated in the Wnt signaling pathway. Conclusion: These findings establish SFRP2 as a prospective gene in CRC, with potential utility in clinical settings, and provide molecular insights into its involvement in colorectal carcinogenesis, offering novel therapeutic approaches for CRC.
Publisher
Research Square Platform LLC
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