High-g Impacts and Vibrational g-Forces can Dislodge Bacillus spp. Spores from Spacecraft Surfaces

Abstract

Abstract Mars spacecraft encounter numerous g-loads that occur along the launch or landing vectors (i.e., called axial vectors) or along lateral off-axes vectors. The goal of this research was to determine if there was a threshold for dislodging spores under brute-force dynamic shock compressional impacts (i.e., henceforth called shock-impacts) or long-term vibrationally induced g-loads that might simulate spacecraft launches or landings profiles. Results indicated that spores of Bacillus subtilis 168 and B. atrophaeus ATCC 9372 were dislodged from ChemFilm-coated aluminum coupons during shock-impact events of 60 g’s or higher. In contrast, the threshold for dislodging B. pumilus SAFR-032 spores was approx. 80 g’s. Vibrational g-loading was conducted at approx. 12–15 g’s (z-axis) and 77 Hz. All three Bacillus spp. exhibited very modest spore dislodgement at 1, 4, or 8 min of induced vibrational g-loads. However, the numbers of spores released depended on the Earth’s g-vector relative to the bacterial monolayers. When the experimental hardware was placed in an ‘Up’ orientation (def. as the spores sat on the upper surface of the coupons and the coupons pointed up and away from the Earth’s g-vector), zero to only a few spores were dislodged. When the experimental hardware was inverted and the coupon surfaces were in a ‘Down’ orientation, the numbers of spores released increased by 20–30 times. Overall, results of both assays suggest that spores on spacecraft surfaces will not likely be be dislodged during nominal launch and landing scenarios, with the exception of jettisoned hardware (e.g., heat shields or backshells) during landing that might hit the Martian terrain at high g’s. Thus, off-nominal landings hitting the Martian surface at > 60 g’s are likely to release spores into the atmosphere and regolith. [Submitted to the journal, Microorganisms, on 08-22-23 for review.]