Complete genome sequence of wheat yellow stripe polerovirus, a new polerovirus infecting wheat (Triticum aestivum) in China

Abstract

Abstract Wheat plants with yellow stripes on their leaves were collected in Tai’an City (Shandong province, China). High-throughput sequencing analysis of the collected plants showed that they were coinfected with wheat leaf yellowing-associated virus (WLYaV) and an unidentified polerovirus. The genome of the unidentified virus, tentatively named “wheat yellow stripe-associated virus” (WYSaV), comprises 5,595 nucleotides and contains six open reading frames (ORFs) initiated by AUG and one ORF initiated by AUA, with a typical polerovirus genome structure. Analysis by sequence alignment showed that WYSaV had the highest sequence identity with wheat yellow dwarf virus (WYDV, a tentative member of the Polerovirus genus), with a nucleotide sequence similarity of 87.3% over the whole genome. Except for P3a and the coat protein (CP), for all deduced proteins between WYSaV and the other poleroviruses, the amino acid identity was < 90%. According to phylogenetic evaluation using the RdRp and CP protein sequences, and the complete genome, WYDV, cereal yellow dwarf virus RPS (CYDV-RPS), CYDV-RPV, and barley yellow dwarf virus-GPV are the phylogenetically closest poleroviruses to WYSaV. Thus, WYSaV is proposed as a new member of the genus Polerovirus.