Abstract
The mechanism of interaction of peripheral tetra-substituted zinc (II) phthalocyanine complex with DNA was evaluated by various procedures in the present study. The DNA interaction capacity of 2, 10, 16, 24-tetrakis (benzhydryloxy) zinc (II) phthalocyanine complex was probed together with the electronic absorption spectra, fluorescence spectra, electrophoresis, thermal denaturation and study of viscosity. DNA binding constant (Kb) and changes in the thermal melting profile of DNA by addition of ZnPc compound revealed that the complex has the capability to attach to CT-DNA by an intercalative binding mechanism. The binding constant Kb that is a crucial parameter for getting information on the binding mechanism, it was estimated to be 1.392 106 M− 1 owing to the electronic absorption analysis and else prominent instrument is fluorescence spectroscopy to clear up the interaction capacity of ZnPc with the DNA. Fluorescence spectrophotometric data provide the evidence that the compound can reacts with DNA thru an intercalative coupling formation pathway. By the outcomes of electrophoresis assays, the declines in the intensity of CT-DNA bands demonstrated that the ZnPc complex interacts with DNA. Further, the data of viscosity measurements demonstrated that the reaction of ZnPc with DNA is most likely via the intercalative binding pathway. All these experimental data revealed that the ZnPc compound interacts with DNA and the compound may be appropriate drugs for the treating cancer due to its DNA interaction activity.