Phytochemical analysis of Stevia rebaudiana using HPLC and GC-MS

Author:

Gunasena M.D.K.M.1ORCID,Senarath W.T.P.S.K.2ORCID

Affiliation:

1. Sabaragamuwa University of Sri Lanka

2. University of Sri Jayewardenepura

Abstract

Abstract Stevia rebaudiana is a medicinal plant rich in important phytochemicals including steviol glycosides. Its’ leaves are the source of non-caloric sweet steviol glycosides and stevioside is the main type. In this study leaves of naturally grown, tissue cultured plants and callus extracts of S. rebaudiana were analyzed using HPLC and GC-MS to determine the possibility of obtaining stevioside and other important phytochemicals through in vitro technology. Phytochemical extraction was done using methanol, evaporated using a rotary evaporator and re-dissolved in acetonitrile: deionized water (3:7) ratio solvent mixture. Stevioside contents were analyzed using HPLC system with C18 column. Methanolic extracts of naturally grown plants, tissue cultured plants and calli were screened using GC-MS and compared. According to HPLC analysis, leaves of tissue cultured plants contained highest amount of stevioside content (11.462 ± 0.03 mg/mL) followed by naturally grown plants and calli. Twenty seven important phytochemicals were identified by GC-MS analysis and out of those fourteen compounds were found to be common in all three samples. Results revealed that in vitro cultures can be used as the better alternative to overcome limitations in conventional propagation and can be used to obtain stevioside and other important phytochemicals instead of natural plants. GC-MS analysis of Stevia extracts revealed the presence of terpenes, fatty acids, aldehydes with biological activities such as antimicrobial, anticancer, anti-inflamatory, antioxidant, antimalarial and etc. Bioactivity of those phytochemicals showed the possible use of Stevia extracts in pharmaceutical, food and perfume industries.

Funder

University of Sri Jayewardenepura

Publisher

Research Square Platform LLC

Reference32 articles.

1. A review on the improvement of stevia [Stevia rebaudiana (Bertoni)];Yadav AK;Canadian journal of plant science,2011

2. Cultivation and uses of stevia (Stevia rebaudiana Bertoni): A review;Hossain MF;African Journal of Food, Agriculture, Nutrition and Development,2017

3. Sterols in Stevia rebaudiana Bertoni;D’Agostino M;Bollettino della Societa italiana di biologia sperimentale,1984

4. GCMS analysis of ester and ethanolic extracts of Stevia rebaudiana (leaves);Maheshwari R;International Journal of Current Advanced Research,2017

5. 73rd Meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), Geneva, Switzerland, 8–17 June 2010;Joint F;FAO JECFA Monographs,2010

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3