Isolation, identification and herbicidal potential of Fusarium avenaceum HY-041 from the Qing-Tibetan Plateau

Abstract

Abstract In order to screen a biocontrol strain with broad-spectrum and high herbicidal activity, potential herbicidal strain HY-041 isolated from the leaves of naturally diseased Cephalanoplos setosum was tested. Based on the morphological characteristics and gene sequence analysis, the fungus was identified as Fusarium avenaceum. Herbicidal activity to 9 target weeds were tested, the results showed that the pathogenicity of strain HY-041 to different weeds in vitro was as follows: Polygonum lapathifolium > Malva verticillata > Avena fatua > Chenopodium album > Polygonum aviculare > Elsholtzia densa > Amaranthus retroflexus> Thlaspi arvense > Galium aparine, the pot experiment showed that the fermentation filtrate was highly pathogenic to C. album, E. densa and A. fatua by spray inoculation. The safety evaluation results showed that the strain was moderately susceptible to Brassica napus L., slightly infected Triticum aestivum L. and Hordeum vulgare, relatively safe to Pisum sativumL. and Vicia faba L.. The scanning electron microscope observation showed that strain HY-041 hyphae destroyed the epidermis tissue, invaded A. fatua tissue from the stoma and parasites, and propagated in the tissue to produce spores. The results showed that strain HY-041 had the potential to develop microbial biocontrol herbicide.