Unravelling protein corona formation on pristine and leached microplastics

Abstract

Abstract Upon entering the environment, the surface properties of pristine plastics are rapidly altered due to interactions with exogenous biomolecules, contaminants, and even microbiota, which ultimately alter their ecological impacts. When present in biological fluids or high protein environments, micro(nano)plastics bind with proteins, which form a protein corona around the particle. Although a significant body of literature exists on protein corona formation on nanomaterials, less is known about how the physiochemical properties of microplastics may influence protein corona formation. This study utilises quantitative proteomics to quantify protein binding to pristine and leached microplastics. Pristine polyethylene (PE) beads (50 and 500µm), polyamide (PA) fibres (100µm), polyethylene terephthalate fibres (500µm), and fragments (<300µm), as well as pristine and leached textile microfibres comprised of PET, recycled PET, PA or cotton were incubated for 24 hours in bovine serum albumin solution (2mg mL-1) to form a protein corona. Protein adsorption to microplastics was dependant on particle surface area to volume ratio but only when additives were absent. For environmentally relevant textile microfibres, cotton microfibres adsorbed significantly more protein than synthetic microfibres. Fourteen-day aqueous leaching increased the surface charge of all microfibres. However, only PA fibres adsorbed significantly higher protein on the leached fibres compared to their pristine counterparts. Overall, the presence of chemical additives in microplastics strongly influenced protein corona formation, and this phenomenon should be incorporated into routine microplastic toxicity assessment.