The association of gene expressions of MAP3K7, NF2, STK3 and STK4 therewith Quantum Resonance Magnetic Analyzer (QRMA) in COVID-19 patients

Abstract

Abstract Objectives COVID-19 is initiated by coronavirus infections and it can principally damage the cell’s function of the lung. Assessment of some important genes may capability more operational administration in patients. Therefore, in the recent study, we investigated mRNA and protein expression of MAP3K7, NF2, STK3 and STK4 in virus-infected patients compared with the control group. In addition, we used Quantum Resonance Magnetic Analyzer (QRMA) in order to analyze blood lipids, basic physical quality, lung function and immune system which are divided into twenty-five different variables in patients compared to healthy individuals. Materials and Methods The statistical population was divided into two different groups: patients and healthy individuals. The Expression of the mRNA level of objective genes was assessed with the SYBR Green Real-time Polymerase Chain Reaction method. The protein level expression of target genes was calculated by Enzyme-linked Immunosorbent Assay. Furthermore, in this study, we use QRMA to analyze some quantities such as blood lipids, basic physical quality, lung function and immune system in patients compared to healthy individuals. Statistical analyses were accomplished with SPSS software (version, 18) and Graph-Pad Prism software (Graph-Pad Prism, version 8.0.1). Results The mRNA and protein expressions level of NF2, MAP3K7, STK3 and STK4 genes in the group of patients decreased significantly compared to healthy individuals. The evaluation of blood lipids, basic physical quality, lung function and immune system in the study by QRMA showed that the Arterial oxygen content Paco2, low-density lipoprotein-C, tonsil immune index and immunoglobulin index were significantly higher in patients group compared to healthy individuals. In contrast, the amount of High-density lipoprotein-C, bone marrow index, respiratory immune index and gastrointestinal immune index were significantly lower in patients group compared to healthy individuals. Conclusions Compared to healthy individuals, the amount of mRNA and protein expressed by NF2, MAP3K7, STK3 and STK4 genes was reduced in COVID-19 patients. In truth, these genes have some critical function in some cellular and sub-cellular pathways. Accordingly, when the expression of genes decreased maybe it can increase the influence of coronavirus. Various mechanisms are involved in COVID-19, the increasing of the amount of the arterial oxygen content Paco2, low-density lipoprotein-C, tonsil immune index, immunoglobulin index and decreasing the amount of the High-density lipoprotein-C, bone marrow index, respiratory immune index and gastrointestinal immune index in COVID-19 patients, can be involved in the effects of coronavirus. Therefore, a deep perception of the relationship between these objective genes and QRMA therewith pathogenesis of COVID-19 infections in a larger population with longer supplements is required.