Emerging evidence for the Prominent correlation between the transcription of P4HB gene and pregnancy outcomes as well as embryonic development

Abstract

Background: Prolyl 4-hydroxylase beta polypeptide, encoded by the P4HB gene, belongs to the protein disulfide isomerases family, implicated in multiple risk factor biological processes related to the occurrence of adverse pregnancy outcomes and embryonic development. Changes in P4HB gene expression play an important role in pathophysiologic processes. Little is known about the regulation of the endogenous P4HB gene, especially through selective splicing. In the present study, we investigated the distribution of P4HB splice variants and the association of P4HB transcription with adverse pregnancy and embryonic development. Methods: Real-time quantitative PCR was used to detect the relative mRNA expression of the P4HB gene in the adverse pregnancy population versus the control population. P4HB gene heterozygous deletion C57BL/6J mice were constructed and analyzed for P4HB difference-associated DEGs using transcriptome sequencing, followed by GO and KEGG enrichment analysis. Sequence characteristics of the predicted encoding protein transcripts of the P4HB gene were analyzed and primers were designed. RT-PCR was applied to identify different transcripts of the P4HB gene in adverse pregnancy and control populations. Results: The increased transcription of P4HB in the adverse pregnancy outcomes population. P4HB (AUC = 0.627) showed high in-sample predictive performance for APO risk in pregnant women. homozygous deletion of the P4HB gene leads to embryonic lethality in mice. Diminished transcription levels of P4HB impacts multiple biological processes according to the study conducted on mice with heterozygous deletion of the P4HB gene. In this paper, we report 17 protein-coding transcripts of the P4HB gene. Expression of transcripts P4HB-201 and P4HB-203 was detected in populations. There was no statistically significant difference in P4HB-203/P4HB-201 expression in the adverse pregnancy group compared to the control group. Conclusions: Our results indicate that there is a clinical correlation between the expression level of the P4HB gene and the occurrence of APOs. Decreased transcription levels of P4HB lead to differences in many biological functions including nucleic acid binding capacity, neurodevelopmental function, substance metabolism. In this study, the presence of P4HB-203 was detected for the first time in human peripheral blood. Transcript P4HB-201 and transcript P4HB-203 were expressed in both adverse pregnancy and control populations.