L. plantarum surface-displayed VP6 (PoRV) protein can prevent PoRV infection in piglets

Abstract

Abstract Porcine rotavirus (PoRV) poses a threat to the development of animal husbandry and human health, leading to substantial economic losses. Among its components, VP6 protein constitutes a major portion of the viral particles and serves as the core structural protein of the virus. In this study, we first developed an antibiotics free and environmentally friendly vector called asd-araC-PBAD-alr (AAPA). Subsequently, we constructed recombinant Lactobacillus plantarum (L. plantarum) strains induced by arabinose to express VP6 and VP6-pFc fusion proteins. Our findings revealed that NC8/Δalr-pCXa-VP6-S and NC8/Δalr-pCXa-VP6-pFc-S strains were capable of enhancing body immunity and protecting suckling mice and piglets from rotavirus infection. The novel recombinant L. plantarum strains developed in this study hold promising potential as oral vaccines to enhance body immunity and present a new strategy for preventing PoRV infection.