Microwave Assisted Extraction of Citrus limetta Peel and assessment of its Bio-Actives Using HRLC-MS/MS

Abstract

Abstract Citrus limetta, often known as sweet lime, is one of India's most important crops due to its high demand in the agro-processing industry. Peel of Citrus limetta has significant levels of polyphenolic chemicals that have pharmacological effects. In current study, the peel is valued for the bioactive compounds it contains. The total phenolic content (TPC) was 16.66 mg GAE/g with an extraction yield of 12.91%. Radical scavenging activity (RSA) was 78.05%, total flavanoid content (TFC) was 9.59 mg QE/g, and total phenolic content (TPC) was 9.59 mg QE/g. Sweet lime peel (SLP) extract was obtained by microwave assisted extraction (MAE) in methanol.LC-MS/MS results showed 18 flavonoid compounds along with polyphenols like rutin, curcumin diglucoside, carotenoid, coumeroic acid, coumaric acids, flavonoid-7-o-glycosides, and Gardenin B that embrace anticancer, antimicrobial, anti-inflammatory, and antitumor activity. This method uses Q-TOF LC/MS with Agilent MassHunter Profiler software to investigate bioactive components in sweet lime peel.