Lysinibacillus macroides 38328, a potential probiotics strain, enhances antioxidant capacity and avian influenza virus vaccine immune response in laying hens

Abstract

Abstract Lysinibacillus sp. is a spore-forming gram-positive bacterium showing excellent adaptive capacity to environment, convenience to storage and antimicrobial properties. Thus, it has appeared a huge potential as probiotics in application, but related studies remain limited. This study was conducted to determine the effects of supplementary Lysinibacillus (L.) macroides 38328 in drinking water on antioxidant capacity and avian influenza virus vaccine immune response of laying hens. Hereon, layers were fed with drinking water with or without supplementary L. macroides 38328 at the dose of 7×109 colony-forming units per milliliter (CFU/mL). The results showed that supplementation with L. macroides 38328 significantly improved the layers’ productive performance while enhancing their antioxidant capacity and immunity. According to the testing results, the rise of serum T-AOC and the downtrend of serum MDA were significant (P < 0.05), while the uptrend of serum SOD concentration was extremely significant (P < 0.01). The bacillus additive also markedly increased the hens’ Stimulation Index and the AIV antibody titer to a trivalent inactivated H5 + H7 vaccine (P < 0.05). Meanwhile, significant improvements (P < 0.05) of productive performance indexes were also noted in treated birds. In conclusion, with its oral safety for laying hens verified, supplementary L. macroides 38328 had positive effects on layers’ heath condition and productivity, indicating great applied potential as a new probiotics strain.