Effect of oleoylethanolamide on systemic inflammation in the development of alimentary-induced obesity in mice

Abstract

Abstract The anorexigenic effect of oleoylethanolamide (OEA, C20H39NO2) was studied in a model of diet-induced obesity in mice. Physiological, biochemical and immunohistochemical methods were used to reveal differences in the changes in the weight of experimental animals, morphological changes in the spleen tissues, as well as changes in the cytokine expression profile in the spleen, blood plasma and in macrophage cell culture. First, it has been shown that a hypercaloric diet high in carbohydrates and cholesterol leads to the development of systemic inflammation accompanied by organ morphological changes and increased production of proinflammatory cytokines. In parallel, the use of OEA reduces the intensity of cellular inflammatory reactions accompanied by a decrease in markers of cellular inflammation and proliferation, such as CD68, Iba-1 and Ki67 in the spleen tissue and stabilizes the level of proinflammatory cytokines (IL-1β, IL6, TNFα) both in animals and in cell culture. In addition, in macrophage cell culture (RAW264.7) it was shown that OEA also suppresses the production of reactive oxygen species and nitrites in LPS-induced inflammation. The results of this study indicate the complex action of OEA in obesity, which includes the reduction of systemic inflammation.