In vitro and In Silico Screening of Antibacterial Compounds from Camellia sinensis Against Bacillus cereus and Escherichia coli

Author:

Boudou Farouk1,BELAKREDAR Amal1

Affiliation:

1. Djillali Liabes University of Sidi-Bel-Abbes, Sidi-Bel-Abbes

Abstract

Abstract In the present study, phytochemical profiling of Camellia sinensis methanolic extract was carried out using liquid chromatography (LC-UV) analysis. Then the antibacterial potential of this extract was evaluated in vitro against Bacillus cereus ATCC 11778 and Escherichia coli ATCC 25922 using the conventional agar well diffusion method. While the compounds identified by LC-UV were tested for their potential DNA-gyrase inhibitory and drug-likeness properties using computer-aided methods. LC-UV analysis revealed the presence of four major compounds namely: Caffeic acid, (-)-Epicatechin, Syringic acid, and (-)-Epigallocatechin gallate. The extract showed powerful antibacterial activity at the concentration of 5000 µg/ml with an inhibitory zone of 25.38 ± 2.75 mm, and 22.78 ± 1.22 mm against B. cereus ATCC 11778, and E. coli ATCC 25922 respectively. (-)-Epigallocatechin gallate exhibited the best docking score (-8.1 kcal mol-1) outperforming the other phenolic compounds but also Clorobiocin and Novobiocin. According to their molecular parameters, all tested compounds revealed an important drug-likeness property by obeying Lipinski's rule of five and the Ghose filter. This analysis revealed that C. Sinensis has strong antibacterial activity due to its high phenolic component content, and it may be considered a promising option for natural plant sources of antibacterial drugs with high value.

Publisher

Research Square Platform LLC

Reference30 articles.

1. Cote, C.K., et al., Bacillus anthracis and other Bacillus species, in Molecular medical microbiology. 2015, Elsevier. p. 1789–1844.

2. Raddadi, N., et al., The most important Bacillus species in biotechnology, in Bacillus thuringiensis biotechnology. 2012, Springer. p. 329–345.

3. Anthrax: A disease of biowarfare and public health importance;Goel AK;World Journal of Clinical Cases: WJCC,2015

4. Potential distributions of Bacillus anthracis and Bacillus cereus biovar anthracis causing anthrax in Africa;Romero-Alvarez D;PLoS neglected tropical diseases,2020

5. Skočková, A., et al., Detection of tetracycline resistance genes in Escherichia coli from raw cow’s milk. Journal of Microbiology, Biotechnology and Food Sciences, 2021. 2021: p. 777–784.

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3