Lactobacillus acidophilus CICC 6075 Attenuates HFD-induced Obesity by Improving Gut Microbiota Composition and Histidine Biosynthesis

Abstract

Abstract This study aimed to investigate the potential anti-obesity efficacy of Lactobacillus acidophilus CICC 6075. The study analyzed metagenomic data from 120 obese and 100 non-obese individuals and found that the abundance of Lactobacillus acidophiluswas significantly higher in normal-weight individuals. High-fat diet (HFD)-induced obese mice were treated with L. acidophilus via daily oral gavage for 12 weeks. After the experiment, the obesity phenotype was assessed, and the fecal 16S rRNA gene sequence was analyzed. Administration of L. acidophilus attenuated excessive weight gain and fat accumulation and maintained the intestinal barrier in HFD-induced obese mice. Sequencing results showed that HFD hindered α and β diversity while reducing the relative abundance of Lactobacillus and norank_f_Muribaculaceaeand significantly increasing the relative abundance of Ilebacterium. L. acidophilus reversed these results and reduced the Firmicutes/Bacteroidetes ratio. Supplementation of L. acidophilusenhanced histidine biosynthesis, inhibited the NF-κB pathway, and significantly reduced the expression levels of inflammatory factors in adipose tissue. These results indicate that L. acidophilus CICC 6075 can significantly improve intestinal microbiota function, promote histidine synthesis, reduce systemic inflammation, and thereby inhibit obesity progression. This suggests that L. acidophilus CICC 6075 may be a good candidate probiotic for preventing obesity.