Abstract
Ectomycorrhizal fungi (EMF) are key symbiotic microbial components for the growth and health of trees in urban greenspace habitats (UGSHs). However, the current understanding of EMF diversity in UGSHs remains poor. Therefore, in this study, using morphological classification and molecular identification, we aimed to investigate EMF diversity in three EMF host plants: Cedrus deodara in the road green belt, and C. deodara, Pinus massoniana, and Salix babylonica in the park road green belt, in Guiyang, China. A total of 62 EMF operational taxonomic units (OTUs) were identified, including 13 EMF OTUs in the C. deodara road green belt, and 23, 31, and 9 EMF OTUs in the park green belts. C. deodara, P. massoniana, and S. babylonica were respectively identified in park green belts. Ascomycota and Basidiomycota were the dominant phyla in the EMF communities in road and park green habitat, respectively. The Shannon and Simpson indices of the C. deodara EMF community in the park green belt were higher than those in the road green belt. EMF diversity of the tree species in the park green belt was P. massoniana > C. deodara > S. babylonica. Differences in EMF community diversity was observed among the different greening tree species in the UGSHs. UGSHs with different disturbance gradients had a significant impact on the EMF diversity of the same greening tree species. These results can be used as a scientific reference for optimizing the design and scientific management of UGSHs.