MicroRNA-193b, a critical factor in cell aging, in human lymphatic endothelial cells

Abstract

Abstract Purpose Recent studies indicate that microRNAs (miRNAs) play essential roles in cellular development, homeostasis, and response. However, the function of miRNA in the senescence of human lymphatic endothelial cells (HLECs) remains unclear. We aimed to find miRNAs related to HLECs’ aging and elucidate miRNAs’ molecular mechanism. Methods HLECs were cultured, and total RNA was harvested from the cells. Then, MiRNA screening was performed, with targeted miRNAs selected using quantitative polymerase chain reaction (qPCR). To evaluate the effect of miR-193b, HLECs were treated with a mimic and inhibitor, and tube formation was investigated. We used a luciferase assay to analyze the expression of CCND1, a well-known gene involved in cell passage, in response to miR-193b. Results Seven miRNAs were primarily screened. Among them, miR-193b-3p showed a high affinity for HLECs. Transfection of the mir-193b-3p mimic decreased cell proliferation and affected tube formation. CCND1 mRNA and protein levels also were decreased by the cell-transfected mir-193b-3p mimic. Conclusion This study provides the first evidence that miR-193b-3p represses cell proliferation and regulates CCND1, which may play an important role in lymphangiogenesis.