Abstract
Cervical cancer is one of the most common malignant cancers in the world and circular RNA plays an important role in the development of human cancer. However, Circ-MEG3 exact function is still unclear. Herein, our results indicate that circ-MEG3 inhibits the growth in vitro and in vivo of human cervical cancer cells. Moreover, we find that circ-MEG3 alters gene expression in human cervical cancer cells. In particular, there were differences in gene expression between rLV-circ group and rLV-circ-MEG3 group, among which 407 genes were up-regulated expression and 8562 genes were down-regulated expression. The important up-regulated genes mainly include CHIC2, EXT1, KDM6A, DDX5, PPARγ, CNBP, PRRX1, BTG1, FBXO11, SRGAP3, SET, PTEN, EIF4A2. The important down-regulated genes mainly include HSP90AB1, CALR, TPM4, HSP90AA1, MYH9, NPM1, HMGA1, CDKN2A, NDRG1, ATP1A1, SDHA, FUS, MYC, SEPT9, CCND3, DNAJB, SND1, CCND1, RPN1, H3F3A. Strikingly, our results show that circ-MEG3 alters cellular functions and cell signaling pathway. The important up-regulated KEGG mainly include p53signaling pathway, Ribosome biogenesis in eukaryotes. The important down-regulated KEGG mainly include cGMP-PKG signaling pathway, Wnt signaling pathway, mTOR signaling pathway, MAPK signaling pathway, Amphetamine addiction. In conclusions, Circ-MEG3 inhibits the progression of human cervical cancer by altering gene expression and related signaling pathways, providing a theoretical basis for clinical diagnosis and treatment of cervical cancer. We will further explore how Circ-MEG3 functions during cervical carcinogenesis and screen its associated important targets.