Isolation, Identification, and Antibiogram of Staphylococcus from Paper Currency and Meat Collected from Butcher House, Restaurant and Abattoir in Jimma Town, South Western Ethiopia

Abstract

Abstract Background: Staphylococcus infections remains a global problem and cause significant morbidity and mortality both in animals and humans. Contaminated meat and paper currency surfaces play a key role in the spread of bacterial infections with antibiotic resistance. Methods: A cross-sectional study was conducted with the objective of isolation and identification of Staphylococcus species from Ethiopian paper currency notes and raw meat handled by the butcher, restaurant and abattoir workers and to determine antimicrobial susceptibility test. A total of 243 samples (135 raw types of meat and 108 paper currency) were examined by biochemical tests for the presence of Staphylococcus species. Results: From a total of 243 collected samples, 26.7% and 64.8 % were found positive isolates of raw meats and paper currency notes respectively. An overall prevalence of 43.6%(106) was recorded. The highest prevalence was observed 39(72.2%) in Hermata merkato kebele whereas the lowest was 3 (11.1%) in Ifabula. Variation within the source of the sample was statistically significant (P<0.05). Of these isolates, 30 selected isolates were tested for antibiotic susceptibility test. Thirty(100%) isolates were susceptible to chloramphenicol. There is no isolate that was sensitive to all four selected antimicrobials (Penicillin G, Gentamycin, chloramphenicol, and Tetracycline). Six isolates of meat (50%) were resistant to penicillin G. which indicates its emerging animal and public health problem. Conclusions: The finding indicated the presence of antibiotics resistance Staphylococcus species contamination of meat and paper currency birr. Awareness creation is paramount important for people working on the food chain to reduce cross contamination of food and prevent foodborne intoxication.