Development and Application of Wide-Genome SSR Molecular Markers of Tapes dorsatus

Abstract

Abstract Background The Tapes dorsatus, is a unique economic benthic animal in the Gulf of Tonkin. The development of microsatellite markers is of great significance for study the genetic improvement, variety identification, phylogenetic development, and conservation of resources the blunt conjugated clam. Methods and results Randomly select 100 pairs of primers for tasting, and 60 pairs of highly polymorphic primers for validation. The average number of alleles (Na) of the highly polymorphic primers was 10.40, the average number of effective alleles (Ne) was 6.16, the average expected heterozygosity (He) was 0.82, and the average polymorphic information content (PIC) was 0.80. Conclusion The 60 microsatellite markers developed in this study showing polymorphism, which can reveal the genetic diversity of the Tapes dorsatus. The research results are helpful for Tapes dorsatus genetic resource protection and genetic breeding research.