Transcriptome analysis of the mechanism to ginsenoside inhibiting on Botrytis cinerea

Abstract

Abstract Background: The control of tomato gray mold mainly relies on chemical fungicides, which can easily cause pesticide residues and environmental pollution. Therefore, a new fungicide for the control of tomato gray mold is urgently needed. Ginsenosides can inhibit the growth of diseases and are safe and non-toxic. To reveal the antibacterial mechanism of ginsenoside against Botrytis cinerea, and to provide theoretical basis for the development of ginsenoside plant pesticide. Results: Ginsenoside showed significant inhibitory activity against B. cinerea, and the inhibition rate reached 95.45% at 1 mg·mL-1. Transcriptome results showed that there were 21 DEGs in the 3 stages, among which 17 genes were upregulated in the treatment group. Bioinformatics analysis showed that the DEGs are mainly related to metabolic pathways such as Metabolism, Cenetic Information Processing, Environmental information Processing and Cellular Processes. Quantitative real-time PCR results demonstrated the accuracy of transcriptome data. Ginsenosides seriously damaged the ultramicrostructure of B. cinerea, resulting in the exosmosis of contents, the formation of a large number of cytoplasm cavities, and the change and degradation of mitochondrial structure. Conclusion: Transcriptomic analysis indicated that ginsenoside may inhibit the growth of B. Cinerea by decreasing the stability of cell membrane or cell wall, and B. Cinerea may respond to the stress caused by ginsenoside by increasing ergosterol synthesis and ABC transporter expression.