Affiliation:
1. SPRI: Shandong Peanut Research Institute
2. Shenyang Agricultural University
Abstract
Abstract
In contrast to its normal-oleic counterpart, high-oleic peanut has better keeping quality and multiple health benefits. Breeding high-oleic peanut through conventional means is a tedious process generally requiring several years. Genome editing, however, may shorten the duration. In this study, node injection method was used to transform normal-oleic Huayu 23, a popular peanut cultivar having dysfunctional FAD2A and functional FAD2B, with CRISPR/Cas9 construct targeting FAD2B, and two T0 seeds with over 80% oleic acid and 442A insertion in FAD2B were obtained. A T1 plant grown from the viable T0 seed produced high-oleic seeds. As a genotype-independent, simple and easy method for peanut genetic transformation, node injection has great potential in functional analysis of genes and peanut varietal improvement.
Publisher
Research Square Platform LLC
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