Synthesis, characterization and antibacterial assays of new N,N 1 -disubstituted 2,2’- dithiodianiline derivatives

Abstract

Abstract A series of novel 1,2-bissubstituted disulfanes bearing beta-amino acid, dihydropyrimidine-2,4-(1H,3H)-dione, hydrazide, hydrazone and azole moieties were synthesized. These disulphides were characterised by spectral and microanalysis data. On the antibacterial evaluation, they were found to have interesting antibacterial properties over a panel of the tested Gram-positive and Gram-negative bacteria: Staphylococcus aureus subs. aureus (ATCC 9144) and zoonotic agent Listeria monocytogenes (ATCC 35152), as well as Gram-negative ones, Escherichia coli (ATCC 13076) and zoonotic agent Salmonella enterica subs. enterica serovar Enteritidis (ATCC 8739). The antibacterial activity was evaluated by determining minimum inhibition (by broth microdilution) and minimum bactericidal (by growth on agar) concentrations. The assessment revealed that MIC values for L. monocytogenes varied between 3.9 and 62.5 µg/mL as well as for S. aureus ranged between 7.8 and 250 µg/mL, with the exception of one compound with much weaker MIC of 500 µg/mL. The MBC values for L. monocytogenes have been found to be of 7.8−250 µg/mL, while S. aureus demonstrated the higher resistance and MBCs varied in the range of 7.8\(\)500 µg/mL. The determined MBC/MIC ratios showed that eleven compounds were classified bactericidal agents for all tested bacteria.