Affiliation:
1. Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine
2. Division of Orthopedic Science, Department of Rehabilitation Science, Kobe University Graduate School of Health Science
Abstract
Abstract
Background: Synovial hyperplasia caused by rheumatoid arthritis (RA), an autoimmune inflammatory disease, leads to the destruction of the articular cartilage and bone. A member of the tumor necrosis factor superfamily, Lymphotoxin-related inducible ligand that competes for glycoprotein D binding to herpes virus entry mediator on T cells (LIGHT) has been shown to correlate with the pathogenesis of RA.
Methods: We used cDNA microarray analysis to compare the expression of genes in RA rheumatoid fibroblast-like synoviocytes with and without LIGHT stimulation.
Results: Significant changes in gene expression (P-values < 0.05 and fold change ≥ 2.0) were associated mainly with biological function categories of glycoprotein, glycosylation site as N-linked, plasma membrane part, integral to plasma membrane, intrinsic to plasma membrane, signal, plasma membrane, signal peptide, alternative splicing, and topological domain as extracellular.
Conclusions: Our results indicate that these gene expression in RA-FLS regulated by LIGHT may be important in the differentiation of several cell types and in cellular functions.
Publisher
Research Square Platform LLC
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