Effects of fullerenol C60 on liver tissue in liver ischemia reperfusion injury in rats undergoing sevoflurane anesthesia Subtitle: Fullerenol C60 in liver ischemia reperfusion injury in rats

Abstract

Abstract The aim of this study was to investigate the effects of fullerenol C60 on rat liver tissue in liver ischemia reperfusion injury (IRI) model undergoing sevoflurane anesthesia to evaluate the ability of nanoparticles to prevent hepatic complications. A total of 36 adult female Wistar Albino rats were divided into six groups each containing six as follows: sham group (Group S), the fullerenol C60 group (Group FC60), the ischemia-reperfusion group (Group IR), and the ischemia-reperfusion-sevoflurane group (Group IR-Sevo), ischemia-reperfusion-fullerenol C60 group (Group IR-FC60), ischemia-reperfusion-fullerenol C60-sevoflurane group (Group IR-FC60-Sevo). Fullerenol C60 100 mg/kg was administrated in Group IR-FC60 and IR-FC60-Sevo. In IR groups 2 hours of ischemia and 2 hours of reperfusion were performed. At the end of the reperfusion, liver tissues were removed for biochemical assays, and histopathological examinations. Hepatocyte degeneration, sinüsoidal dilatation, prenecrotic cell, MN celluler infiltration in the parenchyma were significantly higher in Group IR than all other groups. TBARS level was significantly higher in Group IR than other groups and the lowest TBARS level was in Group IR-FC60 than other groups except Group S and FC60. CAT and GST activities reduced in Groups IR than all other groups. Fullerenol C60 has protective effects against liver IR injury in rats under sevoflurane anesthesia. The use of fullerenol C60 could reduce the adverse effects of IRI and associated costs in liver transplantation surgery.