AURKB is a key to connect oxidative phosphorylation and immune microenvironment in Gastric Cancer.

Abstract

Abstract The oxidative phosphorylation(OXPHOS) is one of the important activities and plays an important role in the development of tumor. This study combines transcriptome and clinical data from gastric cancer(GC) patients from the GEO and TCGA databases. Consensus Cluster Analysis divides GC patients into OXPHOS-high and -low groups. The GSVA analysis displayed significant differences in the immune microenvironment among the different OXPHOS groups. Furthermore, WGCNA was used to screen immune-related core molecules, and analyzed the differentially-Expressed Genes(DEGs) among different subtypes. AURKB was identified as a key molecule linking oxidative phosphorylation and immunity in GC. Subsequently, the biological functions of AURKB in GC were analyzed using transcriptomics and cell experiments. Finally, the link between AURKB and immunotherapy effect in patients with GC was analyzed in combination with multiple immune-related databases. Our study determined that AURKB is involved in the OXPHOS and affects the response to immunotherapy in GC patients. The combination of AZD1152 and targeted Therapy or immunotherapy may be a promising strategy in the treatment of GC.