Abstract
Conventional methods for Lawsonia inermis L. propagation face problems like short viability, disease, pests, and unreliable seed propagation due to natural habitat conditions for large-scale production. In-vitro micropropagation also shows drawbacks in mass-scale production due to poor hardening percentage and more time requirements in the context of producing the desired amount of leaf biomass. Therefore, vegetative propagation via stem cuttings to multiply superior, healthy plants is convenient for achieving increased productivity in terms of leaf biomass. Auxins have a significant effect on the rooting and shooting of cuttings. An experiment was carried out at the Botanic Garden, University of Chittagong, in the southeastern region of Bangladesh to determine the effect of IAA, IBA, and NAA on the vegetative growth potential of stem cuttings of L. inermis following the quick dip method and completely randomized design. Results showed significant variability in root and shoot development, which influences the leaf biomass production of L. inermis cuttings. IAA, IBA, and NAA all had significant effects on shoot and root development on cuttings except in control cuttings, where cuttings developed adequate roots without hormone application. It concludes that IBA-treated cuttings could be considered as a higher amount of leaf biomass producing and more effective than IAA and NAA as propagation material for L. inermis, which may play a vital role in the herbal medicine industry as well as in economically developing countries like Bangladesh. It was recommended to investigate how IAA, IBA, and NAA, together with other plant growth regulators, affect L. inermis's capacity for roots and shooting in producing the greatest amount of leaf biomass.