A novel protocol to establish subarachnoid hemorrhage mouse model with preserved vascular anatomy

Abstract

Abstract This study introduces a novel Circle of Willis perforation (cWp) subarachnoid hemorrhage (SAH) model in mice. Employing Needle Puncture and Filament Perforation through the Common Carotid Artery (CCA), the method replicates the External Carotid Artery (ECA) SAH model's characteristic, preserving all normal anatomical structures. Notably, surgical complexity decreases significantly, highlighting its potential to streamline cWp model production and recommending adoption in the broader SAH research community.