Comparative Analysis of Radiation Response in Chemo-Treated BT20, 4T1 Breast Cancer, and Neuroblastoma Cancer Cell Lines through Single and Multiple Cell Ionization Using Infrared Laser Trapping

Abstract

Abstract The aim of our study was to use laser trapping technique to evaluate the radiation sensitivity of cancer cells, both untreated and chemo-treated. We focused on a specific human breast tumor cell line, called BT20, that was treated with an antitumor compound known as 2-Dodecyl-6-methoxycyclohexa-2, 5-diene-1, 4-dione. We investigated an untreated control group, as well as two groups of BT20 cells that were subjected to different treatment durations. We utilized a high-power infrared laser (at 1064 nm) trap to determine the absorbed threshold ionization energy (TIE) and threshold radiation dose (TRD) for single and multiple cells trapping and ionization. We performed statistical analyses, including descriptive and one-way ANOVA, on the results. We also analyzed the relationship between TIE and TRD to the mass of the individual cells for different hours of treatment, in comparison to the control group. Our findings revealed that both TIE and TRD decreased as treatment duration increased. However, the TRD decreased with mass irrespective of the treatment. Moreover, our analyses consistently showed the same behavior of TRD for single vs. multiple cell ionizations within each group, regardless of treatment. In addition, we conducted a comparative analysis on the effects of radiation dosage in BT20, 4T1 breast cancer, and N2a neuroblastoma cell lines, and discovered that the TRD decreased in mass across all three cell lines.