Identification of RNA Modification-Related Differentially Expressed Genes in Acute Myocardial Infarction: Unraveling Diagnostic Perspectives and Immunological Microenvironment

Abstract

Abstract Acute myocardial infarction (AMI) is a critical cardiovascular disease with significant health implications. This study aims to investigate the role of RNA Modification-Related Genes (RMRGs), which are essential post-transcriptional regulators, in the pathology of AMI. By examining AMI-related datasets (GSE24519, GSE48060, GSE34198), RMRGs were collected from GeneCards and PubMed. The analysis involved enrichment analyses using Gene Expression Omnibus (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA). Additionally, Gene Set Variation Analysis (GSVA) was conducted, and regulatory networks (mRNA-miRNA, mRNA-TF, mRNA-RBP) were constructed. Immune cell infiltration was evaluated through single-sample gene-set enrichment analysis (ssGSEA) and CIBERSORT algorithms. In the AMI Dataset, 131 differentially expressed genes (DEGs) underwent GO and KEGG analyses, revealing significant enrichment in biological processes and pathways such as Allograft rejection, TNF signaling, and autoimmune diseases. GSEA identified gene enrichments in various pathways. Six key genes (APC2, C9ORF72, CDKN1C, GOLGA8A, PHKA2, RPL9) emerged as crucial factors in AMI development, as highlighted by Gene Set Variation Analysis (GSVA). Furthermore, extensive analysis was conducted on immune cell associations and interaction networks. The findings suggest that genes related to RNA modification, especially the identified key genes, significantly contribute to the pathogenesis of AMI. Consequently, this study provides valuable insights into the molecular mechanisms and potential treatments for AMI.