Biosurfactant production by Bacillus cereus GX7 utilizing organic waste and its application in the remediation of hydrocarbon-contaminated environments

Abstract

The use of biosurfactants represents a promising technology for remediating hydrocarbon pollution in the environment. This study evaluated a highly effective biosurfactant strain-Bacillus cereus GX7’s ability to produce biosurfactants from industrial and agriculture organic wastes. GX7 showed poor utilization capacity for oil soluble organic waste but effectively utilized of water soluble organic wastes such as starch hydrolysate and wheat bran juice as carbon sources to enhance biosurfactant production. This led to significant improvements in surface tension and emulsification index. Corn steep liquor was also effective as a nitrogen source for GX7 in biosurfactant production. The biosurfactants produced by strain GX7 demonstrated a remediation effect on oily beach sand, but are slightly inferior to chemical surfactants. Inoculation with GX7 (70.36%) or its fermentation solution (94.38%) can effectively enhanced the degradation efficiency of diesel oil in polluted seawater, surpassing that of indigenous degrading bacteria treatments (57.62%). Moreover, Inoculation with GX7’s fermentation solution notably improved the community structure by increasing the abundance of functional bacteria such as Pseudomonas and Stenotrophomonas in seawater. These findings suggest that the GX7 as a promising candidate for bioremediation of petroleum hydrocarbons.