Affiliation:
1. University of the Punjab
2. University of Central Punjab
3. University of Narowal
4. Government College University
Abstract
Abstract
Bioaerosol contains many pathogenic species of bacteria. The workers of dairy farms are continuously exposed to the farm environment so they are more prone and susceptible to airborne infections. In the current study, different samples from the dairy farm of three regions viz sewerage site, river Ravi and urban areas were collected. The bacterial load was accessed and also some pathogenic bacteria were isolated. The 16S rRNA gene characterization analysis followed by the phylogenetic tree analysis showed 98–99% similarities with the staphylococcus sciuri, bacillus toyogensis and bacillus pulmis. The overall microbial load was higher in open and sewerage site farms as compared to the open farm of river Ravi and urban area farm. Similar trend was observed in closed farms. The 16s rRNA gene characterization and phylogenetic analysis not only help to identify species level identification but also genetic relatedness with other species. So, farm’s worker’s health is more at risk at open and sewerage site farm than river Ravi and urban area farms.
Publisher
Research Square Platform LLC
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