Affiliation:
1. Pohang University of Science and Technology
2. IISER Bhopal: Indian Institute of Science Education and Research Bhopal
Abstract
Abstract
Magnesium oxide nanoparticles (MgO-NPs) are highly versatile and have been extensively utilized in diverse industrial and biomedical applications due to their exceptional physical and chemical properties. However, the potential harms to human health and the environment from their use continue to be of great trepidation. In this study, we delved deep into the intricate molecular mechanisms underlying the detrimental effects of MgO-NPs on the growth and viability of the model yeast Saccharomyces cerevisiae. Our findings demonstrate that as the concentration of MgO-NPs increases, it leads to a dose-dependent reduction in the growth and viability of the yeast cells. We further investigated the underlying mechanisms of MgO-NP toxicity and found that it causes damage to the cell membrane, which in turn triggers an endoplasmic reticulum (ER) stress response. The response to ER stress involves an increase in the expression of genes that play a role in protein folding, maintaining protein quality, and removing misfolded proteins via ER-associated degradation (ERAD). In response to treatment with MgO-NPs, we observed the activation of the cell wall integrity (CWI) pathway, it caused the activation of chitin production genes and an increase in the amount of chitin in the cells. These findings highlight the multifaceted detrimental nature of MgO-NPs, which involve the interplay of various molecular networks and signaling pathways.
Publisher
Research Square Platform LLC
Reference38 articles.
1. Therapeutic and diagnostic potential of nanomaterials for enhanced biomedical applications;Abd Elkodous M;Colloids Surf B Biointerfaces,2019
2. A study of the yeast cell wall composition and structure in response to growth conditions and mode of cultivation;Aguilar-Uscanga B;Lett Appl Microbiol,2003
3. Exploring the nano-fungicidal efficacy of green synthesized magnesium oxide nanoparticles (MgO NPs) on the development, physiology, and infection of carrot (Daucus carota L.) with Alternaria leaf blight (ALB): Molecular docking;Ahamad L;J Integr Agric,2023
4. The efficiency of metal, metal oxide, and metalloid nanoparticles against cancer cells and bacterial pathogens: different mechanisms of action;Alavi M;CMBR,2022
5. Biogenic green synthesis of MgO nanoparticles using Saussurea costus biomasses for a comprehensive detection of their antimicrobial, cytotoxicity against MCF-7 breast cancer cells and photocatalysis potentials;Amina M;PLoS ONE,2020