Affiliation:
1. Wuhan Academy of Agricultule Sciences
2. Wuhan Academy of Agricultural Sciences
3. Wuhan Academy of Agriculture Sciences
Abstract
Abstract
Rhizoctonia solani endornavirus 8 (RsEV8) was isolated from strain XY175 of Rhizoctonia solani. The full-length genome of RsEV8 is 16147 nucleotides (nt) in length and contains a single open reading frame that encodes a large polyprotein of 5227 amino acid. The polyprotein contains four conserved daomains including viral methyltransferase, putative DEAH box helicase, Viral helicase and RNA-dependent RNA polymerase (RdRp). RsEV8 has a shorter 3' leader (58 nt) and a longer 5' trailer (404 nt) sequences. A multiple sequence alignment indicated that the RdRp of RsEV8 has eight typical RdRp motifs. According to the BLASTp result of the nucleotide sequence, RsEV8 shares 39.31% identity to Rhizoctonia cerealis endornavirus-1084-7. Phylogenetic analysis demonstrated that RsEV8 clustered with members of the genus Betaendornavirus.
Publisher
Research Square Platform LLC
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