Oleuropein protects against 5-Fluorouracil-Induced nephrotoxicity and hepatotoxicity in Rats

Abstract

Abstract Purpose The renal and liver dysfunction is considered to be a life-threatening side effect of chemotherapy drugs such as 5-fluorouracil (5-FU). This study was designed to assess the effects of Oleuropein (OLE) against 5-FU-induced renal and liver toxicity. Methods Twenty-four adult Sprague-Dawley rats were randomly divided into 4 groups (N = 6) including the control, 5-FU (100 mg/kg), OLE (200 mg/kg), 5-FU + OLE. After treatment, blood sample were collected for the measurement of biochemical parameters, and the kidney and liver tissues were removed for the determination of inflammatory markers and histological changes. Results 5-FU significantly increased blood urea nitrogen (BUN), creatinine (Cr), uric acid (UA), liver enzyme levels, tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) concentration. Also, histopathological findings of 5-FU showed acute tubular necrosis (ATN) in the renal tissues and coagulative necrosis in the hepatocytes. While the administration of OLE significantly decreased BUN-Cr, liver enzyme levels and inflammation and improved pathological changes. Conclusion Oleuropein exerted hepato-nephroprotective effects against 5-fluorouracil toxicity by the reduction of inflammatory factors.