Unique algorithm for the evaluation of embryo photon emission and viability-Reference-Cited by-同舟云学术

Unique algorithm for the evaluation of embryo photon emission and viability

Published:2023-09-14 Issue: Volume: Page:
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Author:

Berke József¹,Gulyás Istvan¹,Bognár Zoltán²,Berke Dávid³,Enyedi Attila⁴,Kozma-Bognár Veronika⁵,Mauchart Péter⁶,Nagy Bernadett⁶,Várnagy Ákos Várnagy⁶,Kovács Kálmán⁶,Bódis József⁶

Affiliation:

1. National Laboratory on Human Reproduction, University of Pécs, Pécs, Hungary

2. 3. Department of Medical Biology and Central Electron Microscope Laboratory, Medical School, University of Pecs, Pécs, Hungary

3. 5. John von Neumann Computer Society, Multimedia in Education Section, Budapest, Hungary

4. 6. Dennis Gabor University, Institute of Basic and Technical Sciences

5. 2. Dennis Gabor University, Department of Drone Technology and Image Processing Scientific Lab, Budapest, Hungary

6. 7. Department of Obstetrics and Gynecology, Medical Scholl, University of Pecs, Pécs, Hungary

Abstract

Abstract Living cells have spontaneous ultraweak photon emission derived from metabolic reactions associated with physiological conditions. The ORCA-Quest CMOS camera (Hamamatsu Photonics, Japan) is a highly sensitive and essential tool for photon detection; its use with a microscope incubator (Olympus) enables the detection of photons emitted by embryos with the exclusion of harmful visible light. With the application of the second law of thermodynamics, the low-entropy energy absorbed and used by embryos can be distinguished from the higher-entropy energy released and detectable in their environment. To evaluate higher-entropy energy data from embryos, we developed a unique algorithm for the calculation of the entropy-weighted spectral fractal dimension, which demonstrates the self-similar structure of the energy (photons) released by embryos. Analyses based on this structure enabled the distinction of living and degenerated mouse embryos, and of frozen and fresh embryos and the background. This novel detection of ultra-weak photon emission from mouse embryos can provide the basis for the development of a photon emission embryo control system. The ultraweak photon emission fingerprints of embryos may be used for the selection of viable specimens in an ideal dark environment.

Publisher

Research Square Platform LLC

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