Kaistella yananensis sp. nov., a novel indoleacetic acid producing bacterium isolated from the root nodules of Sophora davidii (Franch.) Skeels

Abstract

Abstract A novel endophytic bacterium named BT6-1-3T was isolated from the root nodules of Sophora davidii (Franch.) Skeels growing in Yan’an, Shaanxi Province, China. Cells were Gram-staining-negative, non-motile, oxidase-positive, catalase-positive and did not produce H2S. Strain BT6-1-3T grew at 15–40℃ (optimum 30℃), at pH 6.0–10.0 (optimum pH 9.0) and with 0–1% (w/v) NaCl (optimum 0.5%). The quinone system was menaquinone 6. The major fatty acids present in BT6-1-3T were iso-C11:0, iso-C15:0 and C16:0. The G + C content of genomic DNA was 39.35 mol% by whole genome sequencing. According to the analysis of 16S rRNA gene sequence, the closest relative was Kaistella montana WG4 (97.57%). The genome similarity was calculated using average nucleotide identity and genome-to-genome distance analysis. Both strongly supported that the strain BT6-1-3T belonged to the genus Kaistella as a representative of a new species. Based on phylogenetic analysis, chemotaxonomic data and physiological and biochemical characteristics, strain BT6-1-3T represents a new species of the genus Kaistella and is named as Kaistella yananensis sp. nov. Type strain is BT6-1-3T (= NBRC 115452T = CGMCC 1.60032T).