Whole-genome sequencing of Pseudomonas koreensis isolated from diseased Tor tambroides

Abstract

Abstract BackgroundPseudomonas koreensis have been reported as a pathogen in freshwater fish. Unlike environmental P. koreensis from soil, which has been studied extensively for its role in promoting plant growth, pathogenic P. koreensis from fish has been assessed only to a very limited extent. This is the first draft genome of P. koreensis CM-01 isolated from the internal organs of diseased Tor tambroides.ResultsHerein, we report the morphological and biochemical characteristics, as well as whole genome sequences of a newly identified P. koreensis strain. We achieved a high-quality draft genome of P. koreensis CM-01 with a N50 value of 233,601 bp and 99.5% BUSCO completeness. The total size of P. koreensis CM-01 genome contains 6,171,880 bp and has a G+C content of 60.5%. Besides, the genome sequence includes 5538 protein-coding genes, 3 rRNA genes, 54 tRNAs, and no plasmids were found. Besides these, 39 interspersed repeat and 141 tandem repeat sequences, 6 prophages, 51 genomic islands, 94 insertion sequences, 4 clustered regularly interspaced short palindromic repeats, 5 antibiotic-resistant genes, and 150 virulence genes were predicted in the P. koreensis CM-01 genome. In addition, the assembled CM-01 genome was successfully annotated against the Cluster of Orthologous Groups of proteins database, Gene Ontology database, and Kyoto Encyclopedia of Genes and Genome pathway database. A comparative analysis of CM-01 with three representative strains of P. koreensis revealed that 92% of orthologous clusters were conserved among these four genomes, and only the CM-01 strain possesses unique elements related to pathogenicity and virulence.ConclusionsThis study provides fundamental phenotypic and genomic information for the newly identified P. koreensis strain. Further study will be necessary to fully understand any correlation between the bacteria and the occurrences of diseases in Tor tambroides.