Affiliation:
1. Xuzhou Medical University
2. Affiliated Hospital of Xuzhou Medical University
Abstract
Abstract
Background
Lung cancer is the most common malignant tumors. In the research, we identified a long non-coding RNA(lncRNA) AC100826.1 (simplify to Lnc1), which was highly expressed in non-small cell lung cancer (NSCLC) tissues compared with the paracancerous tissues. we observed the critical role of Lnc1 in regulating the metastasis ability of NSCLC cells.
Methods
RNA sequencing was performed to detect differential expression levels of lncRNAs in NSCLC tissues and its paracancerous tissues. Effects of Lnc1 on cell proliferation, migration were determined by CCK-8, transwell and scratch assays. The xenograft experiment confirmed the effect of Lnc1 on NSCLC in vivo. RT-qPCR and Western blots were performed to determine the expression levels of mRNAs and proteins.
Results
The expression level of Lnc1 was related to multiple pathological results, knockdown of Lnc1 can inhibit the proliferation and metastasis abilities of NSCLC cells. silencing phospholipase C, β1(PLCB1) can reverse the promoting effects of overexpression Lnc1 on NSCLC cells proliferation and migration abilities. Besides, the Rap1 signaling pathway implicated in the regulation of Lnc1 in NSCLC metastasis.
Conclusion
Our results suggest that Lnc1 regulated the metastatic ability of NSCLC cells through targeting PLCB1/Rap1 signal pathway.
Publisher
Research Square Platform LLC