Coronary microthrombi in the failing human heart: the role of von Willebrand factor and PECAM-1

Author:

Giannakopoulos Theodoros1,Richter Manfred2,Kostin Sawa1,Krizanic Florian1,Sasko Benjamin1,Ritter Oliver1,Pagonas Nikolaos1,Kostin Sawa1

Affiliation:

1. Medizinische Hochschule Brandenburg Theodor Fontane

2. Kerckhoff Klinik

Abstract

Abstract Background and aim. The recognition of microthrombi in the heart microcirculation has recently emerged from studies in COVID-19 decedents. The present study investigated the ultrastructure of coronary microthrombi in heart failure (HF) due to cardiomyopathies that are unrelated to COVID-19 infection. In addition, we have investigated the role of von Willebrand factor (VWF) and PECAM-1 in microthrombus formation. Methods. We used electron microscopy to investigate the occurrence of microthrombi in patients with HF due to dilated (DCM, n=7), inflammatory (MYO, n=6) and ischemic (ICM, n=7) cardiomyopathy and 4 control patients. VWF and PECAM-1 was studied by quantitative immunohistochemistry and Western blot. Results. In comparison to control, the number of microthrombi was increased 7-9 times in HF. This was associated with a 3.5-fold increase in the number of Weibel-Palade bodies (WPb) in DCM and MYO compared to control. A 5-fold increase in WPb in ICM was significantly different from control, DCM and MYO. In Western blot, VWF was increased 2-fold in DCM and MYO, and more than 3-fold in ICM. The difference between ICM and DCM and MYO was statistically significant. These results were confirmed by quantitative immunohistochemistry. Compared to control, PECAM-1 was by approximatively 3-fold increased in all groups of patients. Conclusions: This is the first study to demonstrate the occurrence of microthrombi in the failing human heart. The occurrence of microthrombi is associated with increased expression of VWF and the number of WPb, being more pronounced in ICM. These changes are likely not compensated by increases in PECAM-1 expression.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3