Abstract
Recent scientific and technical advancements have resulted in the creation of very sensitive biosensing devices for pathogen detection. Surface plasmon resonance (SPR) has developed as an environmentally benign and effective label-free detection method in clinical research, notably for studying biomolecular interactions such as hemoglobin interactions. SPR can be used to assess blood abnormalities such as anemia, which is characterized by a transitory fall in blood hemoglobin concentration due to a decrease in red blood cell count. When compared to gold film sensors, using silver as the metal material and graphene to improves the sensitivity and gives a more distinct reflectance spectrum. The fundamental goal of this research is to improve hemoglobin detection sensitivity by concentration analysis, hence leading to a better knowledge of blood behavior utilizing SPR. This entails examining SPR reflectivity and calculating electromagnetic field dispersion in multilayer structures using a mechanism for wave propagation in stratified media based on transfer matrices.