Osteogenic promoting effect of inflammatort cytokines in Ossification of the Posterior Longitudinal Ligament

Abstract

Abstract Objective To investigate the effects of IL-6, IL-1β, MCP-1, TNF-α, RANTES and IL-10 on osteogenic differentiation of Ossification of Posterior Longitudinal Ligament (OPLL) cells.Methods: Posterior longitudinal ligament was collected during surgical spinal procedures in 5 patients with OPLL. OPLL cells were isolated by enzymatic digestion of the tissue.The primary OPLL cells were cultured and passed, and the post-passage OPLL cells were subjected to osteogenic culture. In the process of osteogenic culture, 10-40ng/ml concentrations of IL-6, IL-1β, MCP-1, TNF-α, RANTES, IL-10 and all the above factors were added respectively.Alkaline phosphatase (ALP) activity levels of cells were detected After 7 days.Results: Compared with the control group (without adding any cytokine), the activity of ALP of OPLL cells was significantly increased regardless of the concentration of IL-1β, TNF-α, MCP-1, RANTES and IL-10, and the promoting effect of adding IL-1β was the most significant. For the same cytokine, in the concentration range of 10-40ng/ml, the osteogenic differentiation ability of IL-6, IL-1β, TNF-α, MCP-1, RANTES and IL-10 groups was positively correlated with the concentration. Compared with the control group, the osteogenic differentiation ability of OPLL was significantly enhanced after the addition of all the above cytokines, and the activity of ALP was much higher than that of the control group and other single-cytokine intervention groups regardless of the concentration; At different concentrations, when the concentration reached 40ng/ml, the group had the most obvious effect on osteogenic differentiation of OPLL cells.Conclusion: Both pro-inflammatory cytokines (IL-1β, TNF-α, MCP-1, RANTES) and anti-inflammatory cytokines (IL-10) can promote osteogenic differentiation of OPLL cells. In the range of 10-40ng/ml, the concentrations of inflammatory cytokines were positively correlated with the osteogenic differentiation ability of OPLL cells. Various inflammatory factors have a synergistic effect in the osteogenic differentiation of OPLL cells.