Affiliation:
1. Affiliations 1Clinical Medical College, Ningxia Medical University
2. Medical Record Management Department, Gansu Provincial hospital
3. Gansu Provincial hospital
4. First Clinical Medical College of Lanzhou University
Abstract
Abstract
Purpose
This study was designed with the goal of exploring miR-99a expression in T2DM patients suffering from comorbid NAFLD and clarifying the importance of miR-99a in this pathological context.
Methods
A total of 137 subjects were included in this study, including 50 T2DM patients with NAFLD (T2DM + NAFLD group),48 T2DM patients without NAFLD (T2DM group), and 39 healthy subjects (Control group). We measured the levels of IL-6, mTOR and SOD in the serum of the subjects by ELISA. The plasma miR-99a levels was detected by RT-PCR. The correlation between serum miR-99a level and other indicators was analyzed.
Results
Serum miR-99a levels (median 0.79 vs 0.16 vs 0.03, P < 0.001) were significantly lower in the T2DM group than the healthy population and further decreased in the T2DM with NAFLD patients (P < 0.001). After adjusting for age, gender, illness duration and BMI, spearman correlation analysis showed that TG, HBA1c, FPG, HOMA-IR, Hs-CRP, IL-6, HDL-C, mTOR(P < 0.05) remained independently linked with serum miR-99a. And stepwise linear regression analysis showed that HBA1c, IL-6 and mTOR are independent serum miR-99a correlation variables (P < 0.05). Moreover, the ROC results indicated that serum miR-99a has a high diagnostic value for T2DM with NAFLD. In conclusion, serum miR-99a may be utilized as a screening biomarker for T2DM with NAFLD.
Conclusions
These data highlight a potential role for miR-99a as a regulator of the comorbid incidence of T2DM and NAFLD, suggesting that measuring the levels of miR-99a can effectively predict the risk of NAFLD in those with T2DM.
Publisher
Research Square Platform LLC