Affiliation:
1. School of Plant and Environmental Sciences, Virginia Polytechnic Institute and State University
2. virginia tech
Abstract
Abstract
The lack of known cell-type specific marker genes in most plants is a major hurdle for single-cell analysis. To address this challenge, we developed an approach to identify Orthologous Marker Gene groups (OMGs) across monocots and dicots and showed that they are capable of determining cell identities in tomato. This result indicates employing conserved OMGs from reference single-cell maps can annotate cell types in other plant species.
Publisher
Research Square Platform LLC
Reference27 articles.
1. Single-cell RNA sequencing resolves molecular relationships among individual plant cells;Ryu KH;Plant Physiol,2019
2. Single-cell transcriptome atlas and chromatin accessibility landscape reveal differentiation trajectories in the rice root;Zhang TQ;Nat Commun,2021
3. Ground tissue circuitry regulates organ complexity in maize and Setaria;Ortiz-Ramírez C;Science (1979),2021
4. A suberized exodermis is required for tomato drought tolerance;Cantó-Pastor A
5. A single-cell analysis of the Arabidopsis vegetative shoot apex;Zhang TQ;Dev Cell,2021