Identification of a Disulfidptosis-related gene associated with the prognosis of pancreatic cancer

Abstract

Abstract Background: Pancreatic adenocarcinoma(PAAD) is a digestive solid tumor with a poor prognosis among many common cancers. Now a new way of cell death has been discovered. This mode of death is known as disulfide death and may be associated with tumor progression. However, the role of this gene in PAAD and its relationship to prognosis remains unclear. This study aims to explore the prognostic role of disulfide death-related genes in breast cancer and their effects on immunity and interstitium. Result: In this study, PAAD samples from TCGA, GTEx, and GEO databases were used to investigate the expression of 10 disulfide death-related genes and the predictive potential of patients' prognosis and survival. Univariate Cox regression analysis was used to analyze 10 genes, and it was found that NCKAP1 and RPN1 were highly expressed in pancreatic cancer tissues and correlated with the overall survival of patients. Univariate and multivariate Cox regression analysis showed that NCKAP1 was an independent factor affecting the prognosis of patients. This study combined NCKAP1 with some clinical factors to construct an ideal prognostic model. In addition, in our study, NCKAP1 was found to be closely related to cancer immune response, and significantly correlated T-cell infiltration, chemotherapy drug sensitivity, and sulfur metabolic channels. The difference in the expression level of NCKAP1 in pancreatic cancer cells and normal controls was verified by the GEO cohort, which was consistent with the public database TCGA. Conclusion: NCLAP1 may play a role in inducing disulfdptosis and regulating tumor immunity, and can serve as a potential therapeutic target for PAAD.