Novel sustainable Cellulose Acetate based biosensor for Glucose Detection

Abstract

Abstract In this study, green ZnO/polypyrrole (ppy)/cellulose acetate (CA) film was prepared via solvent casting. This film was used as supporting material for glucose oxidase (GOX) to sensitize a glucose biosensor. First, Zinc oxide (ZnO) nanoparticles have been prepared via the green route using olive leaves extract as a reductant. ZnO/ppy nanocomposite has been synthesized by a simple in-situ chemical oxidative polymerization of pyrrole (Py) monomer using FeCl3 as an oxidizing agent. The produced materials and the composite films were characterized using X-ray diffraction analysis (XRD), scanning electron microscope (SEM), Fourier transform infrared (FTIR) and thermogravimetric analysis (TGA). Glucose oxidase was successfully immobilized on the surface of the prepared membrane and then ZnO/polypyrrole/cellulose acetate/GOX composite was deposited on the platinum electrode for determination of the current at different initial concentrations of glucose. Current measurements proved the suitability and the high sensitivity of the constructed biosensor for the detection of glucose levels in different samples. The performance of the prepared biosensor has been assessed by measuring glucose concentration for different samples with known concentrations. The results affirmed the reliability of the developed biosensor towards real samples which suggests the wide-scale application of the proposed biosensor.