Affiliation:
1. Renji Hospital, Shanghai Jiaotong University School of Medicine
Abstract
Abstract
Background
Knee osteoarthritis is a common musculoskeletal disease characterized by degeneration of articular cartilage. Current diagnostic tools are limited to radiographs and physical examination.
Methods
We collected osteoarthritis cartilage tissues of five patients at different OA stage. We performed genome-wide DNA methylation sequencing and proteomic assays. For proteomic data processing, raw data from LC-MS/MS were imported into Proteome Discover 2.4 (Thermofisher) for analysis. For DNA methylation data processing, statistical differences in continuous variables between the two groups were compared by independent-samples t test. The possible causative genes were identified by cross-analysis of the two tests
Results
We identified 13 potential genes as osteoarthritis-related genetic signatures after analysis of the methylation and proteome.
Conclusions
Our study found that the process of knee osteoarthritis may be regulated by a combination of epigenetic and genetic modulation.
Publisher
Research Square Platform LLC
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