Development of wound healing scaffold using ZnO and TiO2 nanoparticles

Abstract

Abstract The goal of this experiment is to produce a nanocomposite porous scaffold, using an electrospinning method that has biological properties to use as a skin tissue engineering (TE) and wound dressing. Polyhydroxyethyle methacrylate (PHEMA), polycaprolactone (PCL), Titanium oxide (TiO2) nanoparticles, and zinc oxide (ZnO) nanoparticles, used to fabricate this scaffold. After the polymers were synthesized, both polymers were dissolved in chloroform, and nanoparticles were added. The diameter of the fibers obtained depends on the amount of addition of each nanoparticle, the injection rate of the device and the voltage, and the distance between the collector and the needle. Scanning electron microscopy (SEM) images showed the morphology of the scaffold and the cells can adhere well to nanofibers. The mechanical property test showed appropriate mechanical properties for this polymeric scaffold. X-ray diffraction pattern (XRD) and Fourier transform infrared spectroscopy (FTIR) results confirm the successful loading of nanoparticles into the polymer scaffold. Other biological features are characterized with, in vivo assay and MTT and antibacterial assay. The results show that this scaffold is suitable to use as a skin tissue scaffold and wound dress.