Efficacy of lyophilized Lactobacillus sakei as a potential candidate for the prevention of carbapenem-resistant Klebsiella infection

Author:

Tajdozian Hanieh1,Seo Hoonhee1,AB RAHIM MD1,Lee Young kyoung1,Kim Sukyung1,Jeong Yoon Kyoung1,ul-Haq Asad1,Lee Saebim1,Kyung Shin Kwak2,Ju Jung-Hyun3,Oh Baek-Rock4,kim Chul Ho4,Song Ho-Yeon5

Affiliation:

1. Soonchunhyang University College and Graduate School of Medical Sciences: Soonchunhyang University College of Medical Sciences

2. Chungnam Techno Park

3. Microbial biotechnology research center

4. Microbiol Biotechnology research center

5. Soonchunhyang University College of Medical Sciences

Abstract

Abstract Background Antimicrobial resistance has been considered one of the greatest threats to human health, according to the World Health Organization (WHO). Gram-negative bacteria, especially carbapenem-resistant Enterobacteriaceae (CRE), drive this alarming trend.Among CRE pathogens, carbapenem-resistant Klebsiella pneumonia (CRKP) has recently been reported as a highly infectious one responsible of a high mortality and morbility in adults and immunocompromised patients. Additionally, CRKP-related infections are challenging to treat, as carbapenems are the last resort of antibiotics. Therefore, developing novel drugs with different mechanism of action from the existing drugs is urgently required to defeat this lethal menace. In these circumstances, probiotics intended for being a potential choice to be a therapeutic candidate and inhibit the pathogens. Thus, our research team has been focusing on probiotics for a long time to develop potential anti-CRKP drug agents. Methods After such efforts, we finally found a novel Lactobacillus sakei PMC104 derived from kimchi, a probiotic strain suitable for treating CRE infection. Next, as part of our expansion into therapeutic development, we did media optimization at food grade and then established a scale-up process to pilot scale. A lyophilizate was then obtained, which was subsequently used in a mouse model infected with CRKP. Results Data showed that treatment with L. sakei powder remarkably diminished the body weight loss, mortality, and illness severity in CRKP-infected mice which shows the preventive effect of our PMC 104 against CRKP infection. Discussion Our results exhibit the potential therapeutic effect of our candidate probiotic strain opposed to the CRKP, advocating that L. sakei can be congested as an antimicrobial candidate for treating CRKP infections. However, extensive studies such as toxicity tests and clinical trials are still needed to develop it as a new anti-CRE therapeutic agent.

Publisher

Research Square Platform LLC

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